Journal: Molecules

Article Title: Natural Polyphenol Corilagin Enhances Osteogenesis and Chondrogenesis Differentiation of Mesenchymal Stem Cells: Implications for Bone and Cartilage Regeneration

doi: 10.3390/molecules31010194

Figure Lengend Snippet: Representative cell morphology of BM-MSCs at the third passage as observed under a light microscope at 4× magnification.

Article Snippet: Primary Bone Marrow-Derived Mesenchymal stem cells from normal human (BM-MSCs, ATCC ® number PCS-500-012TM, Lot: 70017526) and human synovial sarcoma cell line (SW-982, HTB-93 TM , Lot: 63971143) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Light Microscopy

Journal: Molecules

Article Title: Natural Polyphenol Corilagin Enhances Osteogenesis and Chondrogenesis Differentiation of Mesenchymal Stem Cells: Implications for Bone and Cartilage Regeneration

doi: 10.3390/molecules31010194

Figure Lengend Snippet: ( A ) Alizarin red S staining revealed the presence of calcium deposits 21 days after the induction of osteogenic medium (OM) in the presence of CLG at varying concentrations (1 μg/mL, 5 μg/mL, and 10 μg/mL). The scale bar (⎯) represents 200 µm. ( B ) Pellet cultures of BM-MSCs were sectioned and stained with H&E, showing the morphology at 21 days after the induction of chondrogenic medium (CM) with CLG at varying concentrations (1 μg/mL, 5 μg/mL, and 10 μg/mL). The scale bar (⎯) represents 200 µm. ( C ) Pellet cultures of BM-MSCs were sectioned and stained with toluidine blue, showing glycosaminoglycans at 21 days after the induction of chondrogenic medium (CM) with CLG at varying concentrations (1 μg/mL, 5 μg/mL, and 10 μg/mL). The scale bar (⎯) represents 200 µm. ( D ) Pellet cultures of BM-MSCs were sectioned and stained with Alcian blue, showing proteoglycans at 21 days after the induction of chondrogenic medium (CM) with CLG at varying concentrations (1 μg/mL, 5 μg/mL, and 10 μg/mL). The scale bar (⎯) represents 200 µm.

Article Snippet: Primary Bone Marrow-Derived Mesenchymal stem cells from normal human (BM-MSCs, ATCC ® number PCS-500-012TM, Lot: 70017526) and human synovial sarcoma cell line (SW-982, HTB-93 TM , Lot: 63971143) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Staining

Journal: Molecules

Article Title: Natural Polyphenol Corilagin Enhances Osteogenesis and Chondrogenesis Differentiation of Mesenchymal Stem Cells: Implications for Bone and Cartilage Regeneration

doi: 10.3390/molecules31010194

Figure Lengend Snippet: ( A ) The effects of different doses of corilagin (1, 5 and 10 µg/mL) on osteogenic differentiation potentials (Runx2, OPN) of BM-MSCs on day 21. Gene expression of differentiation potentials was assessed using real-time PCR. The bar means SD. Data is shown as mean ± SD. Statistical significance was calculated using a one-way ANOVA test and significance is represented on graphs as * p -value < 0.05. ( B ) The effects of different doses of corilagin (1, 5 and 10 µg/mL) on chondrogenic differentiation potentials (Sox9, Aggrecan) of BM-MSCs on day 21. Gene expression of differentiation potentials was assessed using real-time PCR. The bar means SD. Data is shown as mean ± SD. Statistical significance was calculated using a one-way ANOVA test and significance is represented on graphs as * p -value < 0.05.

Article Snippet: Primary Bone Marrow-Derived Mesenchymal stem cells from normal human (BM-MSCs, ATCC ® number PCS-500-012TM, Lot: 70017526) and human synovial sarcoma cell line (SW-982, HTB-93 TM , Lot: 63971143) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Gene Expression, Real-time Polymerase Chain Reaction